Meno: | Jana |
---|---|
Priezvisko: | Černíková |
Názov: | Live Monitoring of MinION Sequencing Runs |
Vedúci: | doc. Mgr. Tomáą Vinař, PhD. |
Rok: | 2022 |
Kµúčové slová: | MinION, real-time monitoring of sequencing runs, analysis of sequenced data |
Abstrakt: | MinION is a small portable sequencing device that allows the analysis of sequenced data already during the sequencing run. Such analysis can be used for monitoring the sequencing run progress in real-time as the data are produced and therefore allows the user to decide when they have a sufficient amount of data to terminate the run. However, to get the results in real-time, such analysis often requires a powerful computer with GPU support. We have developed a new monitoring tool for SARS-CoV-2 sequencing runs. Our tool enables on-the-fly analysis on a regular desktop computer without GPU support, using a complex computational pipeline involving base calling, debarcoding, sequence alignment, and variant identification. We have tested our monitoring tool on real sequencing runs of SARS-CoV-2 clinical samples. |
Súbory bakalárskej práce:
Autor nedal súhlas so zverejnením svojej bakalárskej práce.
Súbory prezentácie na obhajobe:
Obhajoba.pdf |